Sequence differences in the diagnostic target region of the oocyst wall protein gene of Cryptosporidium parasites.
نویسندگان
چکیده
Nucleotide sequences of the Cryptosporidium oocyst wall protein (COWP) gene were obtained from various Cryptosporidium spp. (C. wrairi, C. felis, C. meleagridis, C. baileyi, C. andersoni, C. muris, and C. serpentis) and C. parvum genotypes (human, bovine, monkey, marsupial, ferret, mouse, pig, and dog). Significant diversity was observed among species and genotypes in the primer and target regions of a popular diagnostic PCR. These results provide useful information for COWP-based molecular differentiation of Cryptosporidium spp. and genotypes.
منابع مشابه
The Cryptosporidium oocyst wall protein is a member of a multigene family and has a homolog in Toxoplasma.
Coccidian parasites are transmitted via a fecal oocyst stage that is exceptionally resistant to environmental stress and harsh chemical treatments, which allows parasites to stably persist outside a host. Because of its oocyst durability Cryptosporidium parvum is a significant water- and food-borne pathogen of humans, as well as animals of agricultural importance. To date, only one apicomplexan...
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ورودعنوان ژورنال:
- Applied and environmental microbiology
دوره 66 12 شماره
صفحات -
تاریخ انتشار 2000